Figure 6.

TBX3 protein levels are upregulated by CDK2. Western blot analyses with antibodies to the indicated proteins was performed using protein extracts from PNT1A and SW1353 cells: (A) Cells synchronized in S-phase for 2 or 4 hrs were treated with either olomoucine (an inhibitor of cyclin A-CDK2) or R03306 (inhibits the mitotic cyclinB1 complex); (B) cells transiently transfected with 50nM siRNAs to CDK2 (siCDK2#1 and siCDK2#2) or the equivalent concentration of control siRNA (siCtrl) for 48 hours; (C) cells synchronized in S-phase were treated with or without olomoucine and/or MG132 (proteasome inhibitor).