Figure 2.

Cell transfection and expression of NF-κB and Lef-1. (A) G418-resistant fibroblast cells clone selection. Small colonies were formed in the presence of G418 on the 7th day of selection (left), whereas significantly larger colonies appeared after 20 days of selection (right). (B) The expression of NF-κB and Lef-1 in the three groups. Significant differences in NF-κB and Lef-1 expression between cells transfected with pcDNA3.1(+)-NF-κB and pcDNA3.1(+)-Lef-1, empty vector or negative control are shown (*P < 0.05 negative control vs. the experimental group and empty vector control). All data are normalized to GAPDH and calibrated based on the negative control, whose expression was considered 1 for all genes. The Y-axis is on a logarithmic scale.