Figure 6.

Expression of Shh and Cyclin D1. The influence of NF-κB and Lef-1 on downstream gene expression. Significant differences in Shh and cyclin D1 expression between cells transfected with pcDNA3.1(+)-NF-κB and pcDNA3.1(+)-Lef-1, empty vector or negative control are shown (*P < 0.05 negative control vs. the experimental group and empty vector control). All data are normalized to GAPDH and calibrated based on the negative control, whose expression was considered 1 for all genes. The Y-axis is on a logarithmic scale.