Skip to main content
. 2015 Nov 13;14(21):3498–3505. doi: 10.1080/15384101.2015.1093707

Table 1.

Primers for real time PCR

Definition Sequence (forward/reverse) GenBank Accession
GAPDH forward primer: 5′-CAATGACCCCTTCATTGACC-3′ NM_002046
reverse primer: 5′-TTGATTTTGGAGGGATCTCG-3′
NF-κB forward primer: 5′ AACAGAGAGGATTTCGTTTCCG 3′ NM_003998
reverse primer: 5′ TTTGACCTGAGGGTAAGACTTCT 3′
Lef-1 forward primer: 5′ TGCCAAATATGAATAACGACCCA 3′ NM_016269
reverse primer: 5′ GAGAAAAGTGCTCGTCACTGT 3′
CEA forward primer: 5′ GCAGTATTCTTGGCGTATCA 3′ M29540.1
reverse primer: 5′ GAGACAAAACAGGCATAGGT 3′
CK7 forward primer: 5′ GGTGGATGCCCTGAATGATG 3′ NM_005556.3
reverse primer: 5′ GTCCAGGGAGCGACTGTTGT 3′
CK14 forward primer: 5′ TCTCGCCTCTCTCTGGTCAT 3′ NM_000526.4
reverse primer: 5′ CCTGATACACAAAAACATCAGGA 3′
CK19 forward primer: 5′ GGACCTGCGGGACAAGATTC 3′ NM_002276.4
reverse primer: 5′ GCAGAGCCTGTTCCGTCTCA 3′
Shh forward primer: 5′ CCCCAAATTACAACCCTGAC 3′ NM_000193.2
reverse primer: 5′ CATTCAGCTTGTCCTTGCAG 3′
Cyclin D1 forward primer: 5′ TAGGCCCTCAGCCTCACTC 3′ NM_016269
reverse primer: 5′ CCACCCCTGGGATAAAGCA 3′