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. 2015 Jul 28;14(18):2881–2885. doi: 10.1080/15384101.2015.1068479

Figure 2.

Figure 2.

(A) Functional N-terminal domain of p53 is required to downregulate SLC7A11 and to promote ferroptosis. (A) Schematic diagram showing the locations and sequences of 2 p53 mutants, p53,25,26,53,54 and p533KR. (B) H1299 tet-on p533KR and p53,25,26,53,54 stable line cells were induced by doxycycline (0.1 µg/mL) and total cell lysate was analyzed by western blots for the expression of MDM2, SLC7A11, p53 and VINCULIN. (C) H1299 tet-on p533KR and p53,25,26,53,54 cells were pre-treated with doxycycline (0.1 µg/mL) for 24 hours and then treated with erastin (10µM); images were taken 40 hours thereafter. (D) Quantification of cell death as shown in (C). *, P <0.01 (Student's t test).