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. 2015 Aug 27;14(20):3231–3241. doi: 10.1080/15384101.2015.1084447

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Figure 5. — Sesn2 supports TNFα+CHX-induced cell death through regulation of XIAP. (A) Sesn2-silencing causes accumulation of IAP proteins. (A) XIAP, cIAP1 and cIAP2 proteins are accumulated in Sesn2-silenced cells as compared to the control. The levels of the indicated proteins in untreated and TNFα+CHX treated cells were determined by immunoblotting. (B) Sesn2 silencing does not affect XIAP mRNA-expression. RNA from Sesn2-silenced or control H460 cells was isolated with Trizol reagent, converted to cDNA and analyzed by qPCR. (C, D) XIAP knockdown restore the levels of TNFα+CHX-induced cell death in Sesn2-silenced H460 cells. (C) Analysis of XIAP protein expression in XIAP-silenced H460 cells. Sesn2-silenced or control H460 cells were infected with lentiviral vector expressing shXIAP and the proteins were analyzed by immunoblotting. (D) Analysis of the effect of XIAP knockdown on TNFα+CHX-induced cell death. Cells were generated as in C and cell death was determined by the analysis of sub-G1 population by flow cytometry.