Figure 2.

Clustering analysis. (Top) Heat map of microarray results comparing RB1-deficient FLC in culture to controls. The results show the expression patterns of genes in control cells cultured for 0 hours (5 lanes), 24 hours (4 lanes), and 48 hours (3 lanes). Z-scores are gene normalized over the time course, using only the control samples. The data is divided into 16 bins. The genes are binned according to their regulation by RB1, employing the S2N metric (combining all time points), with those most derepressed in the absence of RB1 in bin 1 and those most repressed in bin 16. There are 1,000 unique genes per bin, except for bin 16, which has 1,047 genes. See supplemental file S1 for greater detail. (Bottom) Color coding and labeling of the major expression pattern clusters.