Figure 5.

Cycloheximide treatment during stress impairs gonad granule formation. 1-d-old tiar-1::gfp hermaphrodites were grown at 20° in the absence of stress (A, B), or exposed to either heat shock (3 hr at 31°) (C) or starvation (4 hr) in liquid medium (see Materials and Methods) (E). tiar-1::gfp hermaphrodites were also incubated with 30 mM cycloheximide while being exposed to the heat shock (D) or starvation (F). Additionally, tiar-1::gfp hermaphrodites were treated with 15 mM puromycin in the absence of stress (B). After the incubation periods, the animals were mounted and imaged using confocal microscopy. Note, puromycin promotes granule formation in most of the nonstressed animals, while cycloheximide impairs granule formation in most of the stressed animals. Dotted squares indicate the zoomed-in areas; “upper plane” refers to a different confocal plane. ds, distal gonad; oo, oocytes. Arrowheads indicate likely P granules, arrows gonad core granules, and empty triangles oocytes granules. The percentage of animals with the depicted phenotype is shown. Scale bars, 10 µm.