Table 2. Cycloheximide treatment during stress impairs gonad granule formation.

Conditions	Treatment	tiar-1::gfp Animals with Granules
		Gonad core	Oocytes	N
Heat shock	None	100%	100%	40
	Cycloheximide	20%	10%	40
Starvation	None	100%	50%	22
	Cycloheximide	5%	0%	40
No stress	None	3.3%	0%	30
	Puromycin 3h	65.8%	10.5%	38
	Puromycin 4h	75%	55%	20

tiar-1(tn1545) 1-d-old hermaphrodites were exposed to the indicated conditions in liquid medium (see Materials and Methods). Then the animals were mounted and observed by confocal microscopy. The number of animals with visible TIAR-1::GFP granules in the gonad core and oocytes was quantified. The data shown is the average of at least two independent experiments.