Table 3. tiar-2 and tiar-3 are not required for gonad core granule formation under stress.
| Genotype | Animals with Gonad Core Granules | ||
|---|---|---|---|
| No Stress | Heat Shock | Starvation | |
| Wild type | 4.2% (n = 119) | 88.8% (n = 98) | 88.6% (n = 79) |
| tiar-1(tn1543) | 4.3% (n = 46) | 4.8% (n = 42) | 10.8% (n = 37) |
| tiar-1(tm361) unc-4(e120) | 0% (n = 24) | 15.2% (n = 33) | 23.5% (n = 51) |
| tiar-2(tm2923) | 0% (n = 37) | 100% (n = 20) | 100% (n = 24) |
| tiar-3(ok144) | 7.1% (n = 42) | 90.1% (n = 22) | 100% (n = 27) |
| tiar-1(tn1543) tiar-2(tm2923) | 0% (n = 28) | 5.9% (n = 34) | 6.5% (n = 31) |
| tiar-1(tn1543); tiar-3(ok144) | 0% (n = 45) | 9.1% (n = 33) | 23.5% (n = 34) |
| tiar-1(tn1543) tiar-2(tm2923); tiar-3(ok144) | 0% (n = 24) | 15.2% (n = 33) | 9.1% (n = 33) |
1-d-old hermaphrodites of the indicated genotypes were kept under normal conditions or exposed to heat shock (3 hr at 31°) and starvation (4 hr without bacteria). Afterward, their gonads were dissected, fixed, and immunostained for CGH-1 (see Materials and Methods). The number of gonads with visible granules in the core was quantified and the average percentage is shown.