Table 1. Integration datasets analyzed in this work.
Database | Model | Selection | Germ-Line Integration | Stage Injected | Detection Technique | Mapped Sites | Reference |
---|---|---|---|---|---|---|---|
Ds selected | Zebrafish | Yes | Yes | 1-cell | TAIL-PCR | 383 | (Quach et al. 2015) |
NGS | 1355 | This work | |||||
Total | 1685 | ||||||
Ds unselected | Zebrafish | No | No | 1-cell | NGS | 1344 | This work |
Tol2 selected | Zebrafish | Yes | Yes | 1–2 cell | Inverse-PCR | 75 | (Kawakami et al. 2010) |
Yes | Yes | 1–2 cell | TAIL-PCR | 304 | (Kondrychyn et al. 2011) | ||
Total | 379 | ||||||
MMLV | Zebrafish | No | Yes | 1000–2000 cell | NGS | 15,223 | (Varshney et al. 2013) |
Sleeping beauty | mESC | No | N/A | N/A | NGS | 131,594 | (de Jong et al. 2014) |
PiggyBac | mESC | No | N/A | N/A | NGS | 122,667 | (de Jong et al. 2014) |
MMTV | Mouse mammary cells | No | N/A | N/A | NGS | 180,469 | (de Jong et al. 2014) |
TAIL-PCR, thermal asymmetric interlaced PCR; NGS, next-generation sequencing; MMLV, Moloney Murine Leukemia Virus; mESC, mouse embryonic stem cells; MMTV, Mouse Mammary Tumor Virus.