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. 2016 Jan 25;6(4):819–828. doi: 10.1534/g3.115.026401

Figure 4.

Figure 4

Actomyosin contraction is restored in drak mutants by myosin II activation. (A) Anti-Anillin antibody-stained drakdel mutant embryos expressing SqhE21, SqhE20E21, SqhA21, or SqhA20A21. Microfilament rings in drakdel;sqhE21 and drakdel;sqhE20E21 embryos are more rounded and regular in shape than microfilament rings in drakdel;sqhA21 and drakdel;sqhA20A21 embryos. (B, C) Plots of circularity indices of microfilament rings of anti-Anillin antibody-stained drakdel mutant embryos expressing SqhE21 or SqhA21 (B), and drakdel mutant embryos expressing SqhE20E21 or SqhA20A21 (C). The circularity index is higher in embryos expressing SqhE21 compared to embryos expressing SqhA21 (B), and in embryos expressing SqhE20E21 compared to embryos expressing SqhA20A21 (C). Mean ± SD, * P < 0.05, ** P < 0.01, **** P < 0.0001. Data were compared using a two-sided Mann-Whitney test. Scale bars, 20 μm.