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T7E1 assay for the indels induced by CRISPR/Cas9 within unselected and puromycin-selected cells. (A), (B), and (C) represent the indels induced by PPAR-γgRNA/Cas9, ATP5E.gRNA/Cas9, and OVA.gRNA/Cas9, respectively. The numbers at the bottom of the gel indicate the mutation frequency as measured by band relative density.
