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. 2016 Feb 10;6(4):917–923. doi: 10.1534/g3.116.027706

Figure 4.

Figure 4

Puromycin sensitivity after puromycin was added. Most of the cells from the control group died, and a portion of cells in the experimental group were alive after puromycin addition for 2 and 4 d. These results indicated that the CRISPR/Cas9 system worked in DF-1 cells. The control group shows cells transfected with Cas9 only and the corresponding RPG reporter. The experimental group refers to cells transfected with CRISPR/Cas9 and the corresponding RPG reporter.