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. 2016 Feb 10;6(4):917–923. doi: 10.1534/g3.116.027706

Figure 7.

Figure 7

T7E1 assay of nine potential off-target effects. (A) Off-target effect for PPAR-γ.CRISPR/Cas9. One, 3, and 5 indicate the control group without puromycin screening for GNS, EHD3, and ADCYAP1, respectively. Two, 4, and 6 indicate the experimental group with puromycin screening for GNS, EHD3, and ADCYAP1, respectively. (B) Off-target effect for ATP5E.CRISPR/Cas9. One, 3, and 5 indicate the control group without puromycin screening for KCNT2, DAD1, and PITPNM2, respectively. Two, 4, and 6 indicate the experimental group with puromycin screening for KCNT2, DAD1, and PITPNM2, respectively. (C) Off-target effect for OVA.CRISPR/Cas9. One, 3, and 5 indicate the control group without puromycin screening for ABCC9, TXNDC12, and LCP2, respectively. Two, 4, and 6 indicate the experimental group with puromycin screening for ABCC9, TXNDC12, and LCP2, respectively (M, Plus2k marker).