Table 3. Primer sequences for detection of off-target analysis.
| Gene | Forward Primer | Reverse Primer | Product Size |
|---|---|---|---|
| GNS | AGCCCCACAGATAGCTGTC | TTCACGCCAGCCACTCCTTC | 544 bp |
| EHD3 | GAGGTTGTGACTCAGTCAGCAC | CACATACATTATCCGAGCCCG | 627 bp |
| ADCYAP1 | GAAATGGAGCAAACAGAGAG | CGTCCTTCATTTGTACTCAGG | 626 bp |
| KCNT2 | CCTCCCCAACAATCACCTCTTCCC | GCTGGGGAAGAAGCAGACA | 610 bp |
| DAD1 | CTCACACAAGGGCACCTCTG | GATAGCTACGGGGCTTCGTG | 636 bp |
| PITPNM2 | GCTGGATCTGTGCATACAAG | GGGTTCATACATGCCATGAC | 597 bp |
| ABCC9 | GGCTACTTGGGTACTGCACTC | CAGTTGCTGCAAAGATCACGC | 388 bp |
| TXNDC12 | CATGCTGACCCGGAAGTGAC | CCGTACACTGATTGATGTGGTG | 363 bp |
| LCP2 | ACCTGAGCCAAGCCTGACTC | TAGGGAGCTGGATTCATTTTCC | 387 bp |