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Genes near variably methylated regions (VMRs) enrich for relevant functions and certain VMRs were experimentally validated as enhancers. (A) VISTA enhancer element 629 was validated positive by transgenic mouse assay. (B) Gene Ontology term enrichment of genes near fetal brain hypomethylated VMRs. (C) WashU Epigenome Browser view of the methylation landscape around the EVX1 gene. VISTA enhancer element 629 overlaps with VMRs downstream of EVX1.
