Figure 5.

Some nonsilencer miniARSs contained regions that contributed to plasmid partitioning. (A) Diagram of low competitive (white bars) and high competitive (black bars) miniARS fragments examined by standard ARS (autonomously replicating sequence) assays in part (B). The line above each pair of fragments indicates the ORC (origin recognition complex) binding site in the standard orientation used throughout this study—the T-rich strand of the ORC site is on the top strand. (B) ARS assays were performed and presented as described for Figure 3B. Acen, lacking a centromere; Cen, containing a centromere.