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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2016 Mar 3;159:94–101. doi: 10.1016/j.jsbmb.2016.03.002

Figure 4. Effect of 1,25(OH)2D3 supplementation on ROS and monocyte adhesion to endothelial cells in glutathione-deficient HUVEC.

Figure 4

The ROS levels in GCLC knockdown HUVEC treated with 1,25(OH)2VD and AA are shown in panel A. The knockdown efficiency of GCLC siRNA along with ICAM-1 protein expression is shown in panel B. The percentage of monocyte adhesion to the GCLC knockdown HUVEC is shown in panel C. Panel D shows the mRNA levels of GCLC in HUVEC. p<0.05 when untreated control cells (#) were compared with GCLC knockdown AA treated or AA+1,25(OH)2VD treated cells (*). 1,25(OH)2D3 supplementation has no beneficial effects in glutathione deficient HUVEC. Values are mean±SE (n=3).