Extended Data Figure 5. PDAC GEMM longitudinal study.

a, Schematic diagram depicting the spontaneous development and progression of pancreatic cancer in Ptf1a^cre/+;LSL-Kras^G12D/+;Tgfbr2^L/L (PKT) mice and H&E of the pancreas at the indicated time points showing healthy pancreas, PanIN lesions, and PDAC lesions. Scale bars: 100 μm. b–c, Exosomes size (b) and concentration (c) assayed by NanoSight® analysis from the serum of PKT mice (E: experimental, red) and control mice (C: control, blue) at 4, 5, 6,7 and 8 weeks of age. ANOVA, post-hoc Tukey-Kramer test, * P<0.05; 3 technical replicates. d, Graph depicting the time wise progression of tumor volume measured by MRI and the % beads with GPC1⁺ bound crExos in individual PKT mice (blue: tumor volume, red: % GPC1⁺ crExos). e, Percent beads with GPC1⁺ crExos on beads from control mice (n=3) and mice with cerulein-induced acute pancreatitis (n=4). Two-tailed Student’s t-test, ns: not significant; 3 technical replicates. f, Results from ROC curves for percent beads with GPC1⁺ bound crExos, exosomes concentration and exosomes size in 4, 5, 6 and 7 weeks old PKT mice (n=7) vs. control (including age-matched littermate healthy control (n=6) and mice with induced acute pancreatitis (n=4), n=10)). Data is presented as the mean ± standard deviation.