Figure 3.

Vegf-A expression is induced by DNp73β. (A) Vegf-A and Mdm2 levels were determined by real-time qPCR analysis after transfection of indicated plasmids in H1299 cells. (B) Effect of indicated plasmids on Vegf-A promoter luciferase construct (Vegf-A-Luc) was assessed by luciferase assay in H1299 cells. (C) Chromatin immunoprecipitation (ChIP) analyses with anti-p73 and anti-IgG antibodies on promoters of Vegf-A and Mdm2 were carried out using H1299 cells inducibly expressing either TAp73β or DNp73β after 8 hours induction. Real-time qPCR was used to quantify the fragments pulled-down with anti-p73 and IgG antibodies, relative to input. (D) Vegf-A and glut-1 levels were determined by real-time qPCR analysis in wild-type, p53^−/−, TAp73^−/− and DNp73^−/− MEFs upon exposure to 1% O₂ for 4 hours.