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. 2015 Dec 4;15(1):41–51. doi: 10.1080/15384101.2015.1120926

Figure 4.

Figure 4.

Influence of rpL3 on MDM2, pERK1/2 and p21 proteins upon Act D treatment. (A) Calu-6 cells were transiently transfected with pHa-rpL3. Calu-6, Calu-6/rpL3 and rpL3ΔCalu-6 were treated with 5 nM Act D for 24 h or untreated. Protein extracts from the samples were analyzed by western blot with indicated antibodies. Anti-vinculin was used as loading control. (B) Effect of rpL3 on ERK expression upon ERK inhibition. Calu-6 cells were transiently transfected with pHA-rpL3. 24 h later, untransfected and transfected cells were treated with 10 μM of the inhibitor PD18 for 1 and 3 h. Protein extracts from the samples were analyzed by western blot using anti-ERK1/2 and pERK1/2 antibodies. Anti-β-tubulin was used as loading control.