Figure 4.

Inhibition of Eg5 inhibitor III, dimethylenastron, induced G₂/M cell cycle checkpoint in the γ-actin knockdown cells. Cell cycle analysis of SH-EP GFP-β-Itubulin cells transfected with either control (A) or γ-actin siRNA (B) and treated with 0–2 µM dimethylenastron for either 22 h (A(i) and B(i)) or 70 h (A(ii) and B(ii)). Data are mean ± SEM of 3 independent experiments.^*P < 0 .05, ^#P < 0 .005, statistically significant between the drug treated cells and its corresponding drug free cells in G₀/G₁ and G₂/M cell cycle phases. ^≠P < 0 .05, ^≠≠P < 0 .005, statistically significant between the drug treated cells and its corresponding drug free cells in S phase. ^xP < 0 .05, ^xxP < 0 .005, statistically significant between the drug treated cells and its corresponding drug free cells in sub-G₁.