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. 2016 Jan 15;15(1):84–94. doi: 10.1080/15384101.2015.1121322

Figure 5.

Figure 5.

BCL10 regulates ubiquitylation at the sites of DNA damage. (A) Immunodetection of BCL10 and ubiquitin (FK2) in T47D cells exposed or not exposed to IR. Quantification of the average number of cryptic γ-H2AX foci per cells is shown on the right. The right panel shows Pearson's correlation coefficient values for co-localization of ubiquitin and BCL10 with and without IR. (B) Immunodetection of BCL10 and ubiquitin (FK2) in T47D cells transfected with control siRNA (CTL siRNA) or BCL10 siRNA (BCL10 siRNA) and exposed to IR (2 Gy). Arrows indicate the accumulation of ubiquitin in the cytoplasm upon BCL10 depletion. Quantification of the average number of ubiquitin (FK2) foci per cell is shown on the right. (C) Immunodetection of BCL10 and RNF8 or RNF168 in T47D cells transfected with control siRNA (CTL siRNA) or BCL10 siRNA (BCL10 siRNA). Quantification of the average number of RNF8 and RNF168 foci per cell is shown on the right. (D) Immunodetection of BCL10 foci in T47D cells transfected with control siRNA (CTL siRNA), RNF8 siRNA (RNF8 siRNA) or RNF168 siRNA (RNF168 siRNA) and exposed to IR (2 Gy). Quantification of the average number of ubiquitin BCL10 foci per cell is shown on the right.