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. 2016 Jan 15;15(1):84–94. doi: 10.1080/15384101.2015.1121322

Figure 7.

Figure 7.

BCL10 promotes DSB repair and cellular sensitivity to DNA damage. Efficiency of BCL10 cellular depletion on DSB repair in cells transfected with control siRNA (CTL siRNA) or BCL10-specific siRNA (BCL10 siRNA). (A) Cells contain an integrated tandem GFP reporter of HR. The percentage of GFP positive was measured by flow cytometer. Each data point represents the mean ± the SEM of 2 separate experiments. (B) Cells contain an integrated tandem GFP reporter of NHEJ. The percentage of GFP positive was measured as in A. (C) Effect of BCL10 depletion on HR proteins. Cells were treated as in A, laser micro-irradiated and allowed to recover for 1 hr and immunostained with different antibodies as indicated. Representative pictures were shown on the right. The percentage of cells with γ-H2AX/BRCA1, γ-H2AX/RPA, γ-H2AX/RAD51 and γ-H2AX/ CtIP were shown on the right. (D and E) Sensitivity of BCL10 knock down cells to different DNA damaging agents (IR and CPT). Survival of U2OS cells upon BCL10 knockdown (BCL10 siRNA) compared with control (CTL siRNA) in response to (D) IR or (E) CPT. Error bars indicate SEM from 2 independent experiments. The results are normalized to plating efficiency.