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. 2015 Oct 27;14(22):3613–3623. doi: 10.1080/15384101.2015.1100777

Figure 2.

Figure 2.

Depletion of NML in senescent cells promotes escape. (A) A549 cells were treated with 0.1 μM doxorubicin for 7 d and then transfected with NML siRNA for 2 d. NML knockdown in senescent cells was verified by western blot. (B) A549 cells were treated with 0.1 µM doxorubicin for 7 d to induce senescence, followed by transfection with NML or p53 siRNA. Colonies formed over the senescent monolayer were stained after 21 d (C) A549 cells were stably infected with retrovirus expressing TIP5 shRNA, the knockdown efficiency was confirmed by RT-qPCR. Values are mean ± SD of triplicates. (D) Pre-rRNA expression levels in A549 TIP5 knockdown cells were determined by RT-qPCR. Values are mean ± SD of 3 experiments. (E) A549 TIP5 knockdown cells were treated with 0.1 µM doxorubicin for 7 d Colony formation in drug-free medium was determined without re-seeding.