Figure 3.

Ecdysone treatment activates transcription of a limited number of genes in S2 Schneider cells. (A) A set of genes exhibiting activated transcription (more than threefold) in S2 Schneider cells one hour after 20-hydroxyecdysone treatment as revealed by RNA-seq analysis. The lines with genes that have a peak in their expression profile during puparium formation are indicated with a gray color. The names of the genes previously described as participants of ecdysone activation are noted in bold. (B) Transcription activation at DHR3 and Hr4 loci in S2 Schneider cells after one hour of 20-hydroxyecdysone treatment determined by RNA-seq analysis. The coordinates of the x-axis correspond to the BDGP5.78 version of the Drosophila genome. The y-axis represents the read density at the corresponding genome regions.