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. 2015 Dec 23;14(24):3864–3876. doi: 10.1080/15384101.2015.1120914

Figure 4.

Figure 4.

Low thymidine concentration decreased dCTP levels in shErk5 cells. (A) dNTP levels in shCtrl and shErk5 cells after 24 h treatment with 100 µM thymidine. All values are normalized to the asynchronous control condition which has been given an arbitrary value of 1.0 (dotted gray line), n=4. (B) dTTP:dCTP ratio after 18 h treatment with thymidine, n=4. (C) The addition of 1 µM deoxynucleosides (N: dA + dC + dG) rescues dCTP levels and decreases subG1 fraction in shERK5 cells. dNTP levels in shCtrl and shERK5 cells after 18 h treatment with 2.5 mM thymidine with or without the addition of deoxynucleosides. All values are normalized to the asynchronous control condition, which has been given an arbitrary value of 1.0 (dotted gray line), n=4. (D) dTTP:dCTP ratio after 18 h treatment with thymidine or thymidine + deoxynucleosides. (E) Cell viability after 18 h treatment with thymidine or thymidine + deoxynucleosides, n=3. Pools of 2 shCtrl or 2 shERK5 cell lines were used in experiments (A-D). One shCtrl and one shERK5 cell lines were used in experiments (E). Student's t-test *p<0.05.