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. 2015 Oct 6;14(22):3544–3556. doi: 10.1080/15384101.2015.1080399

Figure 1.

Figure 1.

Apoptotic activity was analyzed by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) assay. (A) Positive TUNEL straining (green) was observed under a fluorescence microscope. (B) Quantitative analysis. The number of apoptotic cells was calculated by averaging the number of positive TUNEL signals. *Significant interstrain differences, р < 0.05; #significant differences with the preceding age, р < 0.05. Bar 50 mkm.