Figure 6.

Nutlin-dependent activation of p53 induces differentiation of mESCs. (A) RT-PCR analysis of sox17, gata6 and afp RNA transcripts in mESCs untreated and treated with nutlin (10 μM) for 1, 3 and 5 days, gapdh was used as an internal control. (B) Representative images of mESCs colonies untreated and treated with nutlin (10 μM) for 5 d. Cells were examined using light microscope. (C) Immunofluorescent staining of mESCs with antibody to surface-specific marker SSEA-1 (red) after 5 d of nutlin treatment (10 μM). Nuclei were stained with DAPI (blue). Scale bar 75 μM.