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. 2015 Oct 27;14(23):3698–3712. doi: 10.1080/15384101.2015.1104436

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Figure 2. — Upregulated E2F1 and E2F2 are associated with cleaved caspase-3⁺ neurons at 24 hours after SCI. (A-B) E2F1 and E2F2 are induced in neurons of the ventral horn (VH) gray matter at day 1 after SCI. (C) Most of E2F1⁺ cells (red) in the gray matter were also co-labeled with E2F2 (green), which had neuronal morphology. Arrows indicated E2F1⁺/E2F2⁺ cells with z-stacks (x-z axis, y-z axis). (D-E) Co-localization between cleaved caspase-3 (green) and E2F1 (red) or E2F2 (red) is apparent in the VH at day 1 after SCI. Arrows indicated caspase-3⁺/E2F1-2⁺; Arrowheads indicated caspase-3⁻/E2F1-2⁺. Scale bar is 100 μm.