Figure 1.

CAPNS1 depletion alters breast cancer cells growth on plate and in soft agar. Control and CAPNS1 depleted MCF7 cells (1A) or MCF10AT (1B) were plated on 6 cm dishes at the indicated concentration. Two weeks later, the cells were stained with crystal violet and images were acquired by Epson scanner. Images of representative experiments are shown. Colonies were counted using image J software; the graph reports the average of 3 independent experiments (p values <0.01). Control and CAPNS1 depleted MCF7 cells (1C) or MCF10AT (1D) were resuspended in 0.3 % agar and the indicated number of cells was plated on 1% agar according to standard procedures. Three weeks later colonies were counted. The graphs report the average number of colonies counted in 3 independent experiments (p values <0.01). Representative pictures are shown next to the graphs.