Figure 1.

Cell numbers (A and B) and expression of cellular proliferative and apoptotic markers (C and D) of HepG2 and BeWo cells after 72 or 96 hours of cultivation in different choline and glucose concentrations. LG–LC (black bars): low-glucose, low-choline; LG–HC (hatched bars): low-glucose, high-choline; HG–LC (gray bars): high-glucose, low-choline; HG–HC (open bars): high-glucose, high-choline. Different letters indicate significant difference, P < 0.05; (b) P < 0.1.

Abbreviations: CASP3, caspase 3; KI67, antigen identified by monoclonal antibody Ki-67; PCNA, proliferating cell nuclear antigen.