Figure 1.

Parental U2OS, U20S-p27WT, and U2OS-p27K cellswere grown in culture. After 24 h, DOX (1µg/ml) was added to one set of cultures of U2OS-p27K cells. After another 48 h, BrdU (30 µM) was added to all cell cultures including induced and non-induced p27K, parental U2OS cells and U2OS-p27WT cells. Cultures were incubated with BrdU for 60 min (Panel A) and 24 h (Panel C). Cells in each condition and at the indicated times were removed from plates and processed for cell cycle analysis and BrdU incorporation. In Panel B annexin V staining (Materials and Methods) for cells isolated at each time and condition are shown.