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Parental, U2OS-p27WT, and U2OS-p27K cells were grown and treated as described in Figure 1 with one set of U2OS –p27K cells receiving DOX (1μg/ml) and then cells were incubated for 72 h. BrdU (30 μM) was added at this time for 1 h. Panel A shows picture of the cell populations at 72 h and Panel B shows the BrdU incorporation into the cells in all populations.
