Figure 5.

Parental cells were plated at 1million cells/dish (upper panel) and U2OS-p27K cells were plated at various densities as indicated (lower panel). All cells were put into nocodazole-induced mitotic inhibition as described in Figure 4 and Materials and Methods. Cells were released from inhibition and placed in fresh medium. Cell cycle analysis was performed on cells in mitotic inhibition and 14 h post mitotic release. Pictures show cell density.