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Parental and U2OS-p27K were placed in nocodazole-induced mitotic arrest. Cells were released from the mitotic arrest, p27K had been induced 6 h before release for one culture and at 0, 2, 4, 8, 12 h after release for other cultures. Cells were incubated in medium with BrdU. The percent of cells not entering S phase (DNA synthesis) was determined.
