Table 1.
P(LA-co-3HB) production by E. coli BW25113 and sigma factor deleted strains
| Polymer production (g/l) |
|||||||
|---|---|---|---|---|---|---|---|
| Strain | Genotype | Cell dry weight (g/l) | Total | LA | 3HB | Polymer content (wt%) | LA fraction (mol%) |
| BW25113 | Parent | 9.1 ± 0.4 | 5.3 ± 0.2 | 0.9 ± 0.0 | 4.5 ± 0.2 | 58.3 ± 2.2 | 18.6 ± 0.9 |
| JW1907 | ΔfliA | 8.6 ± 1.3 | 4.9 ± 0.6 | 0.8 ± 0.1 | 4.2 ± 0.7 | 57.2 ± 2.0 | 18.4 ± 4.2 |
| JW3169 | ΔrpoN | 8.2 ± 0.8 | 6.2 ± 0.4 | 1.4 ± 0.1 | 4.8 ± 0.4 | 75.1 ± 3.3 | 26.2 ± 2.5 |
| JW4253 | ΔfecI | 10.7 ± 0.3 | 5.7 ± 0.2 | 0.2 ± 0.1 | 5.6 ± 0.2 | 53.5 ± 2.8 | 3.2 ± 1.4 |
| JW5437 | ΔrpoS | 10.0 ± 0.1 | 5.8 ± 0.1 | 0.6 ± 0.1 | 5.2 ± 0.0 | 57.6 ± 0.9 | 12.3 ± 1.4 |
| JW3169/ pCA24N a | ΔrpoN / Plac | 8.0 ± 0.2 | 6.2 ± 0.3 | 1.3 ± 0.1 | 4.9 ± 0.2 | 77.7 ± 2.2 | 24.4 ± 1.2 |
| JW3169/ pCA24N-rpoNa | ΔrpoN/ Plac::rpoN | 7.2 ± 0.5 | 5.0 ± 0.1 | 0.7 ± 0.1 | 4.3 ± 0.0 | 70.0 ± 4.3 | 15.8 ± 1.4 |
pCA24N: empty vector of ASKA clone.
JW3169 / pCA24N: empty vector introduced into JW3169 (rpoN deletion strain).
pCA24N-rpoN: rpoN gene cloned downstream of Plac in pCA24N.
JW3169 / pCA24N-rpoN: rpoN overexpression plasmid introduced into JW3169 (rpoN deletion strain).
a
100 μM of IPTG added.