Figure 3.

The kinase Slipper and the TNF associated factor dTRAF1 are required for rbf1^D253A-induced overgrowth phenotypes but not for rbf1^D253A-induced apoptosis. (A) Wild type wing and overgrowth wing phenotype observed in some fly expressing rbf1^D253A (adapted from Milet et al. 2014).¹⁰ (B, E) Comparison of notch wing phenotypes distribution between vg-Gal4, rbf1^D253A/+ and vg-Gal4, rbf1^D253A/UAS-RNAi slipper (Wilcoxon test: n = 272, α = 6,0 10^-06, Ws = 4,54) and between vg-Gal4, rbf1^D253A /and vg-Gal4, rbf1^D253A /+; UAS-RNAi dtraf1/+ flies (Wilcoxon test: n = 327, α = 8,1 10^-4, Ws = 3,34). (C, F) Comparison of apoptotic cells numbers in the wing pouch imaginal discs between vg-Gal4, rbf1^D253A/+ and vg-Gal4, rbf1^D253A /UAS-RNAi slipper third instar larvae and between vg-Gal4, rbf1^D253A/+ and vg-Gal4, rbf1^D253A/+; UAS-RNAi dtraf1/+ third instar larvae. Asterisks indicate a statistically significant difference between 2 genotypes (Student's test α < 0.05). For each genotype, quantifications were done for 30 third instar larval wing imaginal discs at least. (D, G) Frequencies of rbf1^D253A-induced ectopic tissue observed in vg-Gal4, rbf1^D253A/+ and vg-Gal4, rbf1^D253A /UAS-RNAi slipper flies and in vg-Gal4, rbf1^D253A/+ and vg-Gal4, rbf1^D253A/+; UAS-RNAi dtraf1/+ flies. Asterisks indicate a statistically significant difference between 2 genotypes (Chi² test α < 0.05). Each experiment presented in B, D, E and G was independently performed 3 times.