Figure 6.

Rbf1-induced apoptosis activates a compensatory proliferation mechanism that depends on slipper and dtraf1 (A, D, G, J) PH3 staining used to visualize the mitotic cells in wing pouch imaginal discs from hs-Gal4/+ or hs-Gal4/+; UAS-rbf1/+ or hs-Gal4/UAS-RNAi slipper; UAS-rbf1/+ or hs-Gal4/+; UAS-rbf1/UAS-RNAi dtraf1 third instar larvae. (B, E, H, K) Visualization of apoptosis cells by TUNEL staining in the wing pouch of imaginal discs from the previously described genotypes. (C, F, I, L) Wing phenotypes observed in some fly from hs-Gal4/+ or hs-Gal4/+; UAS-rbf1/+ or hs-Gal4/UAS-RNAi slipper; UAS-rbf1/+ or hs-Gal4/+; UAS-rbf1/UAS-RNAi dtraf1 genotypes (M) Comparison of apoptotic cells numbers in the wing pouch of imaginal discs from the previously described genotypes. Asterisks indicate a statistically significant difference between 2 genotypes (Student's test α < 0.05). For each genotype, quantifications were done for 30 third instar larval wing imaginal discs at least. (N) Comparison of proliferation percentage in posterior compartment from the genotypes described previously. Asterisks indicate a statistically significant difference between 2 genotypes (Student's test α < 0.05). (0) Frequencies of notches phenotypes observed in genotypes flies described previously. Asterisks indicate a statistically significant difference between 2 genotypes (Chi² test α < 0.05). Each experiment presented in N and 0 was independently performed 3 times.