Table 1.
Expression of MSC and ESC markers in AFSC measured by flow cytometry shown as percent positive cells. Corresponding isotype controls IgG1, IgG3, IgG2a, IgM precede actual markers and were matched across lines tested. AFSC showed predominantly a mesenchymal stem cell phenotype and ESC marker expression pattern inconsistent with pluripotency. However, expression of TRA-1-60, TRA-1-81 and SSEA-4 was detected in AFSC9 p = passage number.
| MSC markes [%] |
ESC markers [%] |
||||||
|---|---|---|---|---|---|---|---|
| AFSC[2] | AFSC[4] | AFSC[7] | AFSC[9] | AFSC[12] | |||
| p4 | p5 | p6 | p3 | p5 | |||
| IgG1κ | 3.87 | 3.92 | 4.05 | IgG1κ | 2.05 | 2.04 | |
| CD44 | 99.92 | 99.95 | 98.17 | Oct3/4 | 1.80 | 1.72 | |
| CD73 | 99.98 | 100.00 | 96.07 | IgG1κ | 2.08 | 2.04 | |
| CD90 | 54.23 | 40.43 | 31.31 | Nanog | 5.71 | 6.01 | |
| CD166 | 99.80 | 99.98 | 62.82 | IgG1κ | 2.06 | 2.14 | |
| CD45 | 5.13 | 26.46 | 5.09 | Sox2 | 32.14 | 36.12 | |
| IgG1κ | 3.32 | 3.45 | 3.70 | IgMκ | 2.09 | 2.00 | |
| CD34 | 18.49 | 20.17 | 9.06 | TRA-1-60 | 40.29 | 1.35 | |
| IgG2a | 3.07 | 3.24 | 6.04 | IgMκ | 2.04 | 2.02 | |
| CD105 | 92.45 | 57.50 | 35.31 | TRA-1-81 | 14.49 | 1.85 | |
| Cy2 2nd | 2.23 | 0.85 | 2.64 | IgG1κ | 2.17 | 2.12 | |
| SSEA-1 | 6.00 | 2.54 | |||||
| IgG3κ | 2.19 | 2.14 | |||||
| SSEA-4 | 86.84 | 28.98 | |||||