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. 2015 Dec 10;15(2):234–249. doi: 10.1080/15384101.2015.1121332

Table 2.

Expression of ESC markers measured by flow cytometry and shown as percent positive cells in early-passage fully reprogrammed retroviral AF-iPSC (19, 20A, D1), episomal AF-iPSC grown in VTN/E8 and episomal AF-iPSC grown in Matrigel/mTeSR-1 conditions. IISH1i-BM1 control iPSC line was included. All lines show ESC marker expression pattern consistent with pluripotency.

  Retroviral [%]
Episomal VTN/E8 [%]
Episomal Matrigel/mTeSR-1 [%]
  IISH1i BM1 12 IISH1i BM1 19 20A D1 IISH1i BM1 19 20A D1
  p16 p10 p20 p5 p5 p6 p17 p5 p5 p4
IgG1κ 2.02 2.06 2.33 2.00 2.06 2.07 2.07 2.04 2.11 2.08
Oct3/4 92.12 95.65 99.60 96.90 98.22 97.23 99.47 99.06 96.59 97.56
IgG1κ 2.04 2.01 2.10 1.98 2.01 2.07 2.05 2.05 2.10 2.01
Nanog 90.24 93.87 96.35 88.21 93.64 88.01 98.97 95.16 95.06 88.11
Sox2 100.00 100.00 99.99 99.97 99.93 99.92 99.95 99.98 99.84 99.73
IgMκ 3.08 3.23 3.17 3.03 3.08 3.11 3.09 3.13 3.12 3.04
TRA-1-60 79.41 65.58 96.76 81.31 76.85 94.84 92.99 78.50 72.21 89.74
IgMκ 3.04 3.31 3.25 2.94 2.99 3.05 3.13 3.03 3.09 3.12
TRA-1-81 27.79 22.67 76.66 27.84 29.20 69.77 29.48 14.06 8.95 27.18
IgG1κ 3.22 3.38 3.04 3.09 3.05 3.02 3.11 3.04 2.98 3.04
SSEA-1 5.90 6.34 7.49 6.39 9.93 11.62 4.11 4.72 5.56 4.78
IgG3κ 3.30 3.40 3.02 3.18 3.06 2.97 3.00 3.07 2.94 3.00
SSEA-4 94.34 99.67 99.96 99.90 99.86 99.90 99.88 99.86 98.81 99.53