Figure 4. Elevated TGF-β1 is associated with systemic tolerance in animals with CNS melanoma.
A) Concentration of TGF-β1 in serum of mice with B16 brain or flank tumors. (B) Representative FACS plots demonstrating the percentage of CD8 T cells represented by the adoptively transferred Pmel population in the spleen and cervical lymph nodes after treatment with LY2157299. (C) Summary graphs showing the percentage and number of adoptively transferred Pmel cells recovered from animals with CNS melanoma after treatment with LY2157299. D) Survival of animals with CNS melanoma treated with the TGF-β signaling inhibitor LY2157299. A–C = 5 animals / group, repeated × 2. D = 10 animals / group, repeated × 1.