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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Clin Cancer Res. 2015 Oct 21;22(5):1161–1172. doi: 10.1158/1078-0432.CCR-15-1516

Figure 6. Combination therapy with LM-based vaccination and focal RT is associated with polyfunctional CD8 T cells, an increased Teff to Treg ratio, and improved APC function.

Figure 6

(A) FACS plots demonstrating the effects of combination therapy on cytokine production from adoptively transferred, tumor-infiltrating CD8 T cells. (B,C) Percentages and numbers, respectively, of CD8 TIL producing single and multiple cytokines (D) Quantification of Treg (FoxP3+,CD4+) in treated animals E) Quantification of CD4 TIL producing IL-2. (F,G) Teff to Treg ratios in TIL, with effectors defined as IFNγ+, or IFNg TNFα double positive respectively. H-J) CFSE dilution of OT-1 T cells cultured with pulsed APC from Spleen, DLN and microglia (CD11b+ CD45-mid). (K-M) Summary graphs of the percentage of OT-1 responders producing IFN-γ when co-cultured with the indicated APC populations. (N) TGF-β1 concentrations in supernatants from J, M. A-G, N repeated × 2, N = 3–5 animals, group, H-M repeated × 1, N = 10 animals / group.