Figure 2.
Comparison of fluorescence-conjugated anti-LC3B staining patterns with those generated by LC3B algorithm that generates yellow for punctate and blue for diffuse LC3B staining through an AQUA program (Methods). Representative examples of breast cancers with high (A, B) and low (C, D) LC3B expression are presented. Actual immunofluorescence is shown in the panels on the left (A, C) and the algorithm-generated images on the right (B, D). Arrows show representative areas of correspondence within each tumor.