Figure 1.

Cytokinesis in suspension produces binucleated cells. (A) Experimental schedule. (B) Frequency of binucleated cells in MRC-5 cultures grown in adherence with or without a 3hr NOC treatment (Adherence) or after mitotic shake off and cytokinesis in suspension (Shake off) with or without a 3hr NOC pre-treatment (see experimental schedule). Binucleated cells were identified by actin and tubulin staining. DNA was counterstained by DAPI. Results shown are the mean ± SEM of 4 independent experiments. **P <0.01 (Student's t-test, vs. untreated). (C) Frequencies of the different mitotic stages after detachment from the substrate with or without a 3hr NOC pre-treatment. The different mitotic stages are depicted in the images. PM: prometaphase, M: metaphase, A: anaphase, T: telophase. Results shown are the mean ± SEM of 4 independent experiments. Scale bar = 5 μm.