Figure 2.

Cytokinesis in suspension produces defective cleavage furrow constriction. (A) Representative images of late telophase cells grown in adherence or cytospun on slides after mitotic shake off and cytokinesis in suspension. Scale bar = 5 μm. (B) Contractile ring width in late telophase cells grown on adherence (Adherence), after cytokinesis in suspension (Shake off) or after cytokinesis in suspension with a 3hr NOC pre-treatment (NOC + Shake off). Late telophase cells were identified by the presence of decondensed chromatin and of a midbody following α tubulin and DNA staining; contractile ring width was measured on phase contrast images. Ten to twenty cells were measured for each condition. **P <0.01 (Student's t-test, vs. adherence).