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. 2015 Dec 22;15(2):274–282. doi: 10.1080/15384101.2015.1127469

Figure 4.

Figure 4.

Binucleated cells induced by cytokinesis in suspension arrest in G2 and accumulate p53. (A) Frequency of cyclin B-positive cells among mononucleated cells grown in adherence (Untreated), after camptothecin treatment (CPT) and in mononucleated or binucleated cells obtained 40 hrs after cytokinesis in suspension (Shake off). For each of the 2 experiments at least 500 mononucleated cells were analyzed in adherence conditions, 200 mononucleated and 50 binucleated cells after cytokinesis in suspension. (B) Representative images of p53 and cyclin B immunostaining in cells grown in adherence (Adherence), after camptothecin treatment (CPT), or in binucleated cells obtained 40 hrs after cytokinesis in suspension (Shake off). (C) The graph shows the mean ± SEM of p53 fluorescence intensity (arbitrary units) in cyclin B positive cells grown in adherence (untreated), after camptothecin treatment (CPT), and in mononucleated or binucleated cells obtained 40 hrs after cytokinesis in suspension (Shake off). For each condition, 2 replicate cultures (at least 50 cells/culture) were analyzed. *P<0.05; **P<0.01 ***P<0.001 (Student's t-test). Scale bar = 5 μm.