Fig 6. Suppressor mutations in FgPRP6.
(A). Schematic drawing of the FgPrp6 protein structure showing the PRP6_N domain and 19 TRR repeats. The lower panel shows sequence alignment of the marked region of FgPrp6 with its orthologs from other fungi and humans. Suppressor mutations identified in FgPrp6 were labelled on the top. The putative hPrp4-phosphorylation sites in hPrp6 were boxed and marked with the letter P underneath. (B). Assays for the localization of FgPrp6- and FgPrp6R230H-GFP fusion proteins. In transformants expressing the FgPRP6-GFP or FgPRP6R230H-GFP construct, fluorescence signals were primarily observed in the nucleus. The R230H mutation had no obvious effects on the expression and localization of FgPrp31. Bar = 20 μm.