(A). Schematic drawing of FgPrp31 (with the NOSIC, NOP, and PRP31_C domains) and sequence alignment of its C-terminal region with its orthologs from M. oryzae (Mo), N. crassa (Nc), S. sclerotiorum (Ss), S. cerevisiae (Sc), S. pombe (Sp), and human (Hs). Blue line indicates the region with known 3-D structures in hPrp31. The R464* and L532P mutations were labelled on the top. Putative hPrp4-phosphorylation sites in hPrp31 are boxed and labelled with the letter P. (B). Three-day-old PDA cultures (upper row) and 2-week-old mating plates (lower row) of suppressor strains S2 and S17, Fgprp4 FgPRP31R464* transformant S2Ma, and Fgprp4 FgPRP31L532P transformant S17Ma. (C). Flowering wheat heads inoculated with the same set of strains examined 14 dpi.